Immunoblots were probed using (A)+(B) anti GST and (C) anti Hsp90 antibodies. 160 and 320 nM) to immobilized Hsp90 (10 g.ml?1). Hsp90 was immobilized onto the surface of a ProteOn? GLM Sensor chip (#176-5012, Bio-Rad, US) and protein conversation was decided at 37C. Sensorgrams are representative of quadruple experiments at varying FN concentrations (E) Saturation … Continue reading Immunoblots were probed using (A)+(B) anti GST and (C) anti Hsp90 antibodies